yacP [2017-8-11 16:0:3]

[SW|ribosome]-associated A‐site endoribonuclease

locus: BSU00970

pI: 7.28

mw: 19.53 kDa

protein length: 170 aa

gene length: 510 bp

function: ribosome-dependent mRNA decay

product: A‐site endoribonuclease

essential: no

synonyms

Genomic Context

categories

• [category|SW 3|Information processing] → [category|SW 3.2|RNA synthesis and degradation] → [category|SW 3.2.4|RNases] → [category|SW 3.2.4.2|Endoribonucleases]

Gene

Coordinates: 116,025 → 116,537

The protein

[SW|Domains]

• N-terminal NYN domain [pubmed|28363943,17114934]
• C-terminal flexible RNA-binding domain [pubmed|28363943]

Structure

• [PDB|5MQ8] [pubmed|28363943]

Expression and Regulation

Operons

• genes: [gene|132E91DB8C81CD8C4F5160B2C068B74DA10AE224|gltX]-[gene|5B9D5DA130DC3654F386684156BDC350DD05DB60|cysE]-[gene|E5826A99769E2FE45959A9D623D6467A8DDDEB81|cysS]-[gene|031FDF8443A8A4D5B042B7591EBE2E6C74F07702|mrnC]-[gene|8CB595F84D00D1262EB13E6CF47BE6F00EDE16A5|yacO]-[gene|172652156ABFE72D5BAAC22729CE17CA947538B5|yacP]
  description: [Pubmed|7510287]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|7510287], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

  regulatory mechanism

  • [regulon|T-box|T-box]: transcription antitermination, overlaps a transcription terminator upstream of [gene|5B9D5DA130DC3654F386684156BDC350DD05DB60|cysE], in [regulon|T-box|T-box]

  regulation

  • expression transiently increases in the forespore [Pubmed|22848659]
  • the [SW|T-box] RNA is degraded by [protein|872CCB5A49C9000BD95E4B0472556D5F60F7D7A4|RNase Y] [pubmed|29794222]

Biological materials

Mutant

• MGNA-B885 (yacP::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1884 NBRP B. subtilis, Japan]

References

Reviews

• 28396493

Original publications

• 19258532,28363943,17114934